How to grow magic mushrooms from spores (2024)

Growing mushrooms from spores is a captivating and rewarding process that allows enthusiasts and growers to experience the complete life cycle of these unique organisms. Spores serve as the primary means through which mushrooms reproduce, offering a more advanced method compared to using mycelium or cultivation kits. While it provides a deeper understanding of mycology, to grow magic mushrooms from spores can be more intricate. This article provides a comprehensive guide on how to grow mushrooms from spores, optimized for SEO with relevant cannabis-related keywords.


What is a spore?

A spore is a biological reproductive unit essential for the life cycle of fungi, including mushrooms. These microscopic cells possess the ability to grow into a new organism without the need to fuse with another cell. Spores are resilient, durable, and light, facilitating their dispersion through air or water.

Characteristics of spores

  • Resilient: Ability to withstand adverse conditions.
  • Durable: Can survive long periods awaiting favorable growth conditions.
  • Light and small: Facilitates dispersion through air or water.

In fungi, spores form in specialized structures called basidia, located in fruiting bodies on the mushroom cap’s underside. Once mature, spores detach and disperse in the environment, initiating a new life cycle under favorable conditions.

Obtaining mushroom spores

To acquire mushroom spores, growers must follow a sterile and careful process. Select a mature, healthy mushroom with a well-developed cap for successful spore collection.

Materials needed to grow magic mushrooms from spores:

  • Mature mushrooms
  • Aluminum foil or glass
  • Sterile cultivation bags
  • Disposable gloves
  • Face mask
  • Disinfectant solution


  1. Area preparation: Disinfect all work surfaces and wash hands. Place aluminum foil or glass in the disinfected area.
  2. Spore harvesting: With gloves and a mask for sterility, cut the mushroom stem. Place the cap, gills facing down, on the aluminum foil or glass.
  3. Wait: Allow the mushroom cap to remain on the foil or glass for 12 to 24 hours. During this period, spores will drop, forming a spore print on the chosen material.
  4. Collection and storage: Once spores are printed, cut and store the paper or glass with spores in a sterile cultivation bag, then label properly to identify it.

Additionally, spore vials can be found in various online stores dedicated to mushroom cultivation.

Germinating mushroom spores

The germination of mushroom spores is a key process for successful fungus cultivation. Quality spores are essential, obtained either through purchase or controlled collection.


Spore preparation:

  • Hydrate if purchased dry by soaking in sterile distilled water for a few hours.
  • Inoculation: Use a spore solution to inoculate the chosen substrate.

Creating a sterile environment

  • Perform the process in a clean, draft-free area to avoid contaminants.
  • Use gloves and a mask throughout to maintain sterility.

Incubation to grow magic mushrooms from spores

  • Place incubated Petri dishes in a dark location.
  • Maintain a temperature between 21-25°C for optimal germination.
  • Monitor spore germination and mycelium growth, ensuring the medium stays moist but not saturated.The germination duration varies depending on mushroom variety, ranging from several days to two weeks. Once germinated and significant mycelium development is observed, they are ready for transfer to the cultivation substrate.

Preparation of materials and space

Proper preparation of materials and space is crucial for success in cultivating mushrooms from spores. The importance of selecting the right substrate and methods for maintaining a sterile and aseptic environment is emphasized.

Substrate selection

Choosing the appropriate substrate is essential to provide the necessary nutrients for mushrooms during their growth. Compost, rich in organic nutrients that promote rapid mycelium colonization, is a viable option. Alternatively, pasteurized straw, lighter in weight, also provides an optimal environment for mushroom development. Substrates such as manure or compost mixtures with vermiculite enhance moisture retention and facilitate fruiting.


Sterilization is important to prevent contamination by competing organisms. Substrates can be sterilized through various processes such as a water bath, reaching high temperatures to destroy potential pathogens.

Tools and the workspace must be disinfected with appropriate solutions such as 70% alcohol or diluted bleach. Moreover, handling spores and substrate should take place in as aseptic an environment as possible, ideally using a laminar flow hood or, alternatively, an inoculation box to minimize exposure to airborne contaminants.

How to inoculate magic mushroom spores

Inoculating psilocybin spores is the first step to successfully grow bmagic mushrooms from spores. This process requires precision and knowledge of proper techniques and environmental conditions to achieve germination.

Spore sowing techniques

Spore sowing is the method by which spores are introduced into a sterile environment to promote mycelium growth. Spore syringes can be used to inject directly into the substrate or culture media such as grains or agar. Another option is to make spore prints on aluminum foil and then scrape them onto the substrate. To maintain sterility, remember to work under aseptic conditions, such as inside a cultivation box.

Environmental conditions for germination

To germinate spores and develop mycelium, optimal conditions must be provided. The temperature should be controlled, usually between 21-24°C, and high relative humidity is required. Cleanliness and sterility are equally important to prevent contamination from other fungi and bacteria. Additionally, a nutrient-rich substrate is crucial, often including grains, compost, or specialized mushroom soil mixtures. Darkness is preferable during the initial germination stage, followed by the introduction of light to stimulate fruiting.

Golden Teacher Spore Syringe

Golden Teacher Spore Syringe

18,90 €

Golden Teacher Spores Vial

Golden Teacher Spores Vial


18,90 €

Microscopic Spore Study Kit (Tupper Included)

Microscopic Spore Study Kit (Tupper Included)


43,90 €

Use of syringes and vials: tips

When cultivating mushrooms from spores, syringes and vials are essential tools. Precision and sterility are crucial to ensure cultivation success.

Syringe preparation:

  • Ensure the syringe is sterilized before use.
  • Before inoculation, shake the syringe to evenly distribute the spores in the solution.
  • Use disinfectant alcohol to clean the needle between each inoculation to prevent contamination.

Use of spore vials:

  • Sterilize the syringe needle with a flame.
  • Clean the vial septum with alcohol before inserting the needle.
  • Inject the spores contained in the vial into the chosen substrate.
  • After use, store the vials in a cool, dark place.

Inoculation tips:

  • Inoculate the substrate in a clean environment to avoid contaminants.
  • Distribute the spore solution evenly along the substrate.
  • Minimize the substrate’s exposure time to air to reduce the risk of contamination.

Patience is key; mycelium requires time to develop before mushroom fruiting can take place.

How to preserve mushroom spores

When storing mushroom spores, the priority is to keep them dry and protected from the elements. An effective method is the use of desiccant paper, which absorbs moisture and facilitates preservation. Spores are placed on the paper and then sealed in plastic bags, preferably vacuum-sealed, to minimize exposure to air and moisture.

Keeping spores at a low temperature in a refrigerator can extend their viability. However, for longer periods, freezing provides the best solution, protecting them from degradation for months or even years. Spores should be placed in appropriate containers before placing them in the freezer to avoid the formation of ice crystals.

It is crucial to avoid direct contact with sunlight and heat sources, as they can damage the viability of the spores. Dark containers or wraps that block direct light are highly recommended.

Proper preservation of spores is essential to maintain their ability to germinate when deciding to grow magic mushrooms from spores.

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How to grow magic mushrooms from spores (2024)
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